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Image Search Results
Journal: Drug Design, Development and Therapy
Article Title: Eucommia bark (Du-Zhong) improves diabetic nephropathy without altering blood glucose in type 1-like diabetic rats
doi: 10.2147/DDDT.S98558
Figure Lengend Snippet: Effect of Du-Zhong on phosphorylated Smad 2/3 level in renal tissues of rats. Notes: ( A ) Effects of treatments on p-Smad2/3 in renal tissues of rats. ( B ) STZ-diabetic rats were dosed by oral gavage once daily for 20 days with 1 g/kg Du-Zhong (STZ + DZ). Normal or STZ-diabetic rats received the vehicle treatment at the same volume of vehicle used to prepare the test solution. Ratio of p-Smad2/3/actin is expressed as the mean ± standard deviation (n=4 per group) in each column. *** P <0.001 compared with data obtained from vehicle-treated normal Wistar rats (Wistar). ## P <0.01 compared with data obtained from vehicle-treated STZ-diabetic rats (STZ). Abbreviations: DZ, Du-Zhong; p-Smad2/3, phosphorylated Smad2/3; STZ, streptozotocin.
Article Snippet: Membranes were blocked with 5% nonfat dry milk in Tris-buffered saline Tween (20 mmol/L Tris, pH 7.6, 137 mmol/L NaCl, and 0.1% Tween 20) for 3 hours at room temperature, followed by an overnight incubation at 4°C with polyclonal antibodies against rat transforming growth factor-beta 1 (TGF-β1) (Abcam plc, Cambridge, UK; Cat No ab66043), connective tissue growth factor (CTGF; Abcam plc; Cat No ab6992), signal transducers and activators of transcription 3 (STAT3; Abcam plc; Cat No ab50761),
Techniques: Standard Deviation